Mitochondria are arranged as a complex network in contact with other cell compartments. Confocal microscopy of JC-1 stained cells allows a precise definition of spatial organization of mitochondria together with analysis of their function, as revealed by the mitochondrial potential. In this panel a 3D reconstruction of an area of the same image is reported. Red voxels represent the high-energized mitochondrial area. From here (more pictures available.